Detection range: 96T7Î¼g/L -200Î¼g/L Purpose: This kit is used to determine the content of cystatin C (CysC) in pig serum, plasma and related liquid samples. Experimental principle This kit uses the double antibody sandwich method to determine the level of porcine cysteine â€‹â€‹protease inhibitor C (CysC) in the specimen. The microporous plate was coated with purified porcine cysteine â€‹â€‹protease inhibitor C (CysC) antibody to prepare a solid phase antibody, and cysteine â€‹â€‹protease inhibitor C (CysC) was sequentially added to the microcapsule of the coated monoclonal antibody. The HRP-labeled cystatin C (CysC) antibody was then combined to form an antibody-antigen-enzyme-labeled antibody complex, which was thoroughly washed and then primed with TMB. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with the cysteine â€‹â€‹protease inhibitor C (CysC) in the sample. The absorbance (OD value) was measured at 450 nm using a microplate reader, and the concentration of porcine cysteine â€‹â€‹protease inhibitor C (CysC) in the sample was calculated from a standard curve.
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